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. Author manuscript; available in PMC: 2013 Jul 19.
Published in final edited form as: Cell Host Microbe. 2012 Jul 19;12(1):86–96. doi: 10.1016/j.chom.2012.05.012

Fig. 3. Cell surface LRP1 is elevated by HCMV and released to the medium as infection progresses.

Fig. 3

(A) LRP1 is transiently elevated after infection: Whole cell lysates (WCL) and isolated plasma membrane proteins (PM) were assayed by Western blot for LRP1, β-actin and insulin receptor (IR) at the indicated times after mock infection or infection (5 IU/cell).

(B) LRP1 is elevated after infection with UV-inactivated virus: Whole cell lysates were assayed by western blot for the viral IE1 protein and isolated plasma membrane proteins were assayed at the indicated times for LRP1 and IR or at the indicated times after mock infection or infection (5 IU/cell) with HCMV or UV-inactivated HCMV (UV HCMV).

(C) Accumulation of soluble LRP1 (sLRP1): At the indicated times after infection, soluble LRP1 (sLRP1) released into the medium was immunoprecipitated with LRP1 antibody, captured with protein A beads, then assayed by Western blot for LRP1 (top panel) or lipoprotein lipase (LPL) (bottom panel).

(D) sLRP1 levels in the presence of a metalloproteinase-inhibitor: HCMV infected cells (5 IU/cell) were treated with GM6001 (10 µM) at 48 hpi, and LRP1 levels in the medium and at the PM were determined 24 h later by western blot.