Fig. 3.

Expression analyses of selected ESTs under osmotic stress. (A) Northern blot analysis. Total RNA was denatured, transferred to Hybond-N⁺ nylon membranes and hybridized with DIG-labeled probes. (B) Expression analysis by RT-PCR. LEA, late embryogenesis abundant family protein; PEAMT, phosphoethanolamine methyltransferase; ARF, ETTIN-like auxin response factor; CIP, chloroplast inositol phosphate; XTH, xyloglucan endotransglucosylase/hydrolase; DHN, dehydrin; LOX, lipoxygenase; FNR, ferredoxin-NADP(H) oxidoreductase. C, unstressed control; 1 d, 2 d, 3 d, 5 d and 6 d indicate days of osmotic stress.