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. 2012 Jun 19;62(2):186–195. doi: 10.1270/jsbbs.62.186

Table 7.

Features of EST-SSRs and polymorphism information in 16 tea accessions

Marker name SSR motif Position of repeat motifsa Approximate size range (bp) No. of accessions with amplification Number of transferable species No. of locib No. of alleles Heterozygosityc PIC valued
He Ho
MSE0019 (ac)23(tc)12 5′ 105–150 16 13 m
MSE0021 (tc)20(ta)10 5′ 265–310 13 7 s 10 0.80 0.46 0.78
MSE0022 (tc)19(ta)9 5′ 165–210 16 14 s 14 0.89 0.88 0.88
MSE0023 (tc)13, (tc)7 unknown 180–240 16 12 s 8 0.83 0.63 0.81
MSE0024 (ag)11 5′ 255–285 16 14 s 9 0.82 0.56 0.80
MSE0025 (tc)14 unknown 260–305 16 14 m
MSE0026 (ag)7, (ag)6 5′ 275–300 16 14 s 7 0.79 0.56 0.75
MSE0027 (tc)19 5′ 105–135 15 6 s 5 0.56 0.47 0.52
MSE0029 (ag)14, (ag)7 5′ 365–340 16 11 m
MSE0030 (tc)11 5′ 245–270 16 14 s 10 0.78 0.81 0.75
MSE0035 (tc)13 5′ 210–250 16 13 s 10 0.82 0.81 0.80
MSE0037 (tc)12 TR, 3′ 200–250 16 14 m
MSE0038 (ag)8 5′ 300–320 16 13 m
MSE0039 (ag)21 unknown 135–180 16 14 m
MSE0040 (tc)18 5′, TR 125–155 16 14 s 9 0.73 0.75 0.71
MSE0042 (tc)16 unknown 100–115 16 12 s 8 0.77 0.50 0.74
MSE0043 (ta)7, (ag)10 unknown 170–210 16 12 s 10 0.79 0.69 0.77
MSE0044 (ta)11, (ag)6 5′ 120–145 16 10 s 9 0.75 0.56 0.72
MSE0045 (ag)14 unknown 215–230 16 14 s 6 0.75 0.88 0.71
MSE0047 (tc)13 5′ 245–275 16 14 s 11 0.79 0.75 0.77
MSE0049 (ag)14 unknown 220–250 16 14 s 10 0.84 0.81 0.83
MSE0050 (tc)14 unknown 265–285 16 14 s 11 0.85 0.88 0.83
MSE0051 (tc)15 5′ 185–215 16 14 s 11 0.87 0.81 0.86
MSE0052 (tc)9 tr 260–285 16 13 s 12 0.86 0.81 0.85
MSE0053 (tc)16 5′ 250–275 16 12 s 11 0.83 0.81 0.81
MSE0054 (tc)15 5′ 165–195 16 14 m
MSE0055 (ta)6 unknown 235–265 16 14 s 3 0.22 0.25 0.21
MSE0056 (tc)10 5′, tr 220–240 13 13 s 6 0.75 0.77 0.71
MSE0058 (tc)12 unknown 185–275 16 14 m
MSE0059 (gaa)11 5′ 195–225 16 14 s 7 0.71 0.63 0.67
MSE0061 (tc)9 5′ 135–165 16 13 m
MSE0062 (ag)18 tr 110–140 16 14 s 9 0.84 0.81 0.82
MSE0063 (ag)9 5′, tr 230–255 16 14 s 10 0.79 0.81 0.77
MSE0066e (tc)4, (tc)4, (tc)3, (tc)4, (tc)3 5′, tr 240–260 16 5 s 1 0.00 0.00 0.00
MSE0067 (tc)18 5′ 135–165 16 4 s 11 0.88 0.69 0.87
MSE0068 (tc)9 5′ 255–275 16 14 s 10 0.80 0.88 0.77
MSE0069 (tc)17 unknown 195–225 15 11 s 11 0.85 0.40 0.83
MSE0070 (tc)8,(tc)6 5′ 125–140 16 11 s 6 0.72 0.56 0.68
MSE0071 (tc)14 5′ 110–135 16 13 s 8 0.80 0.63 0.78
MSE0072 (tc)14 5′, tr 295–320 16 14 s 9 0.81 0.75 0.79
MSE0074 (tc)7 unknown 200–210 16 13 s 4 0.62 0.56 0.54
MSE0075 (tc)12 unknown 140–200 16 14 m
MSE0076e (ca)3, (ac)4, (tat)3, (gta)3, (tgg)3 tr 240–245 16 13 s 3 0.36 0.19 0.33
MSE0077 (tc)10 5′ 120–140 16 14 s 8 0.68 0.56 0.64
MSE0078 (ag)16 5′ 140–160 16 14 s 7 0.80 0.69 0.77
MSE0079 (ag)16 5′ 100–115 16 14 s 11 0.76 0.81 0.74
MSE0080e (ac)4, (ta)4, (tca)3, (tc)5 unknown 225–245 16 13 s 5 0.52 0.38 0.48
MSE0081 (tc)9 5′ 100–140 16 8 s 9 0.82 0.31 0.79
MSE0082 (ta)13 unknown 155–185 16 13 s 8 0.81 0.81 0.78
MSE0083 (tct)6 5′ 235–265 16 14 s 9 0.84 0.69 0.83
MSE0084 (tc)16 5′ 395–420 16 14 s 11 0.83 0.81 0.81
MSE0087 (ag)13 5′ 265–285 16 14 s 7 0.79 0.75 0.76
MSE0088 (tc)9 unknown 185–195 15 3 s 4 0.46 0.33 0.42
MSE0089 (ag)7 5′, tr 290–310 16 14 s 7 0.61 0.63 0.59
MSE0094 (ta)8 3′ 180–220 16 14 s 8 0.70 0.56 0.67
MSE0096 (tc)12 3′ 235–260 16 14 s 11 0.74 0.75 0.72
MSE0098 (cca)6 tr 245–270 16 14 s 7 0.62 0.81 0.58
MSE0099 (ag)6 5′ 285–300 16 14 s 7 0.73 1.00 0.70
MSE0100 (tc)15 5′ 235–260 15 7 s 10 0.86 0.93 0.84
MSE0101 (cca)6 5′ 240–270 16 14 s 7 0.77 0.75 0.73
MSE0102e (tc)5(ctc)3, (tc)4, (tc)3 tr 305–345 16 12 s 9 0.78 0.63 0.76
MSE0103 (tc)14 5′ 170–195 16 13 s 8 0.74 0.81 0.72
MSE0106 (tc)6 unknown 145–175 16 14 s 8 0.80 0.44 0.77
MSE0107 (tc)8 5′, tr 290–315 15 14 s 10 0.78 0.87 0.76
MSE0108 (tc)6(ta)8 unknown 245–270 16 14 s 9 0.79 0.81 0.77
MSE0109 (tc)10 unknown 105–125 13 5 s 6 0.67 0.31 0.63
MSE0112e (ag)5, (ag)3, (ag)3, (tg)3 unknown 280–285 16 14 s 2 0.48 0.56 0.37
MSE0113 (tc)14 5′ 350–380 16 0 s 9 0.84 0.75 0.82
MSE0114 (tc)14 unknown 195–205 16 2 s 5 0.61 0.94 0.53
MSE0116 (tc)10 5′ 175–195 16 14 s 6 0.65 0.38 0.61
MSE0117e (aca)3, (ag)4, (ag)3(tg)3 tr, 3′ 105–125 16 14 s 7 0.59 0.31 0.55
a

5′, 5′-UTR; 3′, 3′-UTR; tr, translated region.

b

s, single locus; m, multi-locus.

c

He, expected heterozygosity; Ho, observed heterozygosity.

d

PIC, polymorphism information content.

e

All SSR motifs in these markers are less than 6 times repeats, but these markers were included in the analysis, because the total number of repeats are more than 10.