Figure 2. Expression of c-kit, SOX2, and Nanog in cells under cardiosphere and monolayer culture conditions.

A) Compared to the baseline (red line in top histogram), flow cytometry analysis showed that the expression of c-kit in cardiac outgrowth cells was increased after 3 days culture under cardiosphere condition (black line), but decreased under monolayer condition (green line). B) Quantitative data from 6 separate experiments using different human cardiac outgrowth cells. Quantitative qRT-PCR analysis of 4 separate RNA samples from different human cardiac outgrowth cells showed that the expression of SOX2 (C) and Nanog (D) is also significantly higher in cells cultured under cardiosphere than monolayer conditions.