Table 1. Effect of histone lysine mutations on the growth and DNA damage sensitivity of dia2Δ cells.

Mutant	Growth defect with dia2Δ	DNA damage sensitivity with dia2Δ
H3K56R	++	++
H4K5,12R	+	++
H4K5,8,12R	synthetic lethal	n/a
H4K8R	+	++
H4K16R	−	−
H4K8,16R	+	++
H4K91R	−	−
H3K9R	−	+
H3K14R	−	+
H3K9,14R	−	+
H3K9,14,18,23,27R	+	+

The dia2Δ mutant, each histone mutant and their corresponding double mutant, containing both the dia2Δ mutation and the indicated histone mutation, were assayed for cell growth and sensitivity towards the DNA damaging agent camptothecin (CPT). A ‘−’ represents no effect on growth or DNA damage sensitivity over the individual single mutants. A ‘+’ represents a synthetic effect in growth on regular media (YPD) or DNA damage sensitivity (growth on medium containing CPT). More+s indicate a more dramatic synthetic effect in comparison with other strains tested. Note that the dia2Δ H4K5,8,12R mutant is synthetic lethal, and therefore, DNA damage sensitivity could not be assessed (n/a).