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. 2012 Jul 26;7(7):e41272. doi: 10.1371/journal.pone.0041272

Figure 2. Mutant alleles restore invasive growth in wildtype strain and invasiveness correlates with increased FLO11 mRNA.

Figure 2

Mutations from invasive descendants of glutamine- and ammonium-limited populations were reconstituted in the wildtype strain (A) and tested for invasive growth (B). Distribution of strains with phoT672A (RB389), sfl1Δ (RB595), PTR3647::C…N (RB567), gdhD206E (RB484), gap1Δ (RB318) (C). mRNA levels for FLO adhesion genes (italic) and amino acid permease genes (italic bold) are shown for glutamine-limited mutants Ptr3647::C…N and Sfl1Q320STOP after growth to mid-exponential phase in liquid YPD (D). Presented are log2-transformed ratios of transcripts. The color code bar illustrates fold-change in mRNA in mutants relative to the wildtype strain CEN.PK113-7D. For the entire clustering of genes with more than 2-fold altered mRNA levels see Fig. S2.