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. 2012 Jul 26;7(7):e41272. doi: 10.1371/journal.pone.0041272

Figure 7. DIP5 and GNP1 are essential for biofilm formation and DIP5 depletion abolishes pseudohyphal differentiation of Sfl1Q320STOP.

Figure 7

Biofilm formation on a polystyrene surface was tested in the Sfl1Q320STOP trp1 background (RB52) of Sfl1Q320STOP dip5 (RB317), Sfl1Q320STOP gnp1 (RB196) and compared to ∑1278b strain 10560-2B, ∑1278b dip5 gnp1 (RB157) and the wildtype strain CEN.PK110-16D. After 24 hours growth, in either synthetic complete 0.1% glucose or synthetic complete 2.0% glucose, biofilms were visualized by crystal violet staining and then washed three times (A). Homozygous diploid strains in the Sfl1Q320STOP background were incubated on low-ammonium SLAD medium for 3 days at 30°C to test for pseudohyphal growth (B).