Table 2. S. cerevisiae strains and populations used in this study.

Strain	Genotype	Reference/source
Wildtype CEN.PK113-7D	MAT a	[47]
CEN.PK113-5D	MAT a ura3-52 (generated from progenitor CEN.PK113-7D)	[47]
CEN.PK110-16D	MATα trp1	[47]
10560-2B (Σ1276b)	MAT a ura3-52 leu2::hisG his3::hisG	[48]
THY.AP4	MAT a ura3 leu2 lexA::lacZ::trp1 lexA::HIS3 lexA::ADE2	[46]
THY.AP5	MATα leu2 trp1 his3 loxP::ade2	[46]
RB1	MAT a sfl1Q320STOP pho90T672A	this study
RB3	MAT a PTR3647::CWNKNPLSSIN	this study
RB12	MAT a Δgap1	this study
RB16	MAT a gap1S388P mep2G352S	this study
RB17	MAT a Δgap1 gdh1D206E	this study
RB18	MAT a	this study
RB20	MAT a sfl1Q320STOP pho90T672A ura3	this study
RB52	MATα sfl1Q320STOP trp1	this study
RB157	∑1276b MAT a ura3-52 leu2::hisG his3::hisG dip5:::KanMX gnp1::loxP	this study
RB184	MAT a sfl1Q320STOP pho90T672A ura3 flo11::KanMX	this study
RB196	MATα sfl1Q320STOP trp1 gnp1:: KanMX	this study
RB197	MAT a sfl1Q320STOP pho90T672A ura3 gnp1::KanMX	this study
RB199	MAT a sfl1Q320STOP pho90T672A ura3 ssy1::KanMX	this study
RB201	MAT a sfl1Q320STOP pho90T672A ura3 cyr1::KanMX	this study
RB209	MATα sfl1Q320STOP trp1 dip5::KanMX	this study
RB210	MATα sfl1Q320STOP ura3 mep2::KanMX	this study
RB317	MAT a sfl1Q320STOP pho90T672A ura3 dip5::KanMX	this study
RB318	MAT a ura3-52 gap1	this study
RB389	MAT a ura3-52 pho90T672A	this study
RB428	MAT a PTR3647::CWNKNPLSSIN ura3	this study
RB457	MAT a PTR3647::CWNKNPLSSIN ura3 flo11::KanMX	this study
RB460	MAT a PTR3647::CWNKNPLSSIN ura3 tec1::KanMX	this study
RB461	MAT a PTR3647::CWNKNPLSSIN ura3 tpk2::KanMX	this study
RB465	MAT a PTR3647::CWNKNPLSSIN ura3 cyr1::KanMX	this study
RB468	MAT a PTR3647::CWNKNPLSSIN ura3 dip5::KanMX	this study
RB469	MAT a PTR3647::CWNKNPLSSIN ura3 gnp1::KanMX	this study
RB472	MAT a PTR3647::CWNKNPLSSIN ura3 gcn4::KanMX	this study
RB474	MAT a PTR3647::CWNKNPLSSIN ura3 mss11::KanMX	this study
RB475	MAT a sfl1Q320STOP pho90T672A ura3 gcn4::KanMX	this study
RB477	MAT a PTR3647::CWNKNPLSSIN ura3 ssy1::KanMX	this study
RB484	MAT a ura3-52 gdh1D206E	this study
RB486	MAT a sfl1Q320STOP pho90T672A ura3 ptr3::KanMX	this study
RB488	MAT a sfl1Q320STOP pho90T672A ura3 mss11::KanMX	this study
RB492	MAT a PTR3647::CWNKNPLSSIN ura3 ptr3::KanMX	this study
RB531	MAT a sfl1Q320STOP pho90T672A ura3 gap1	this study
RB538	MAT a sfl1Q320STOP pho90T672A ura3 tpk2::KanMX	this study
RB540	MAT a sfl1Q320STOP pho90T672A ura3 tec1::KanMX	this study
RB547	MAT a PTR3647::CWNKNPLSSIN ura3 gap1	this study
RB567	MAT a ura3-52 PTR3647::CWNKNPLSSIN	this study
RB595	MAT a ura3-52 sfl1Δ	this study
RB598	∑1276b MAT a dip5::KanMX can1::STE2p-SpHIS5 lyp1::STE3p-LEU2 his3::HisG leu2 ura3	[34]
RB599	∑1276b MAT a gnp1::KanMX can1::STE2p-SpHIS5 lyp1::STE3p-LEU2 his3::HisG leu2 ura3	[34]
RB600	∑1276b MAT a gap1::KanMX can1::STE2p-SpHIS5 lyp1::STE3p-LEU2 his3::HisG leu2 ura3	[34]