Figure 6. Cytokine production in LPS-stimulated peritoneal macrophages and phagocytosis function of control mice and mice with high-fat diet induced obesity, administered or not B. uniformis CECT 7771.

SD: standard diet group (control) (n = 6); SD+B: standard diet group receiving a daily dose of 5.0×10⁸ CFU B. uniformis CECT 7771 by gavage for 7 weeks (n = 6); HFD: high fat diet group (n = 6); HFD+B: high fat diet group receiving a daily dose of 5.0×10⁸ CFU B. uniformis CECT 7771 by gavage for 7 weeks (n = 6). In the cytokine production study, peritoneal macrophages were stimulated with purified lipopolysaccharide (LPS) from S. enterica serotype Typhimurium (Figure 6A). Non-stimulated peritoneal macrophages were evaluated as controls of basal cytokine levels. In the phagocytosis study (Figure 6B), evidence of oxygen-radical production by macrophages was determined by the NBT test after in vitro interaction with a bacterial extract. Figure 6A: TNF- α and IL-10 cytokines produced by LPS-stimulated macrophages; Figure 6B: % NBT (+) cells. Data are expressed as mean and standard deviation of duplicate measurements determined in two independent experiments. Statistically significant differences of data are established at P<0.05.