Figure 8. Influence of stool samples from mice fed standard diet or high-fat diet, supplemented or not with B. uniformis CECT 7771, on cytokine production by peritoneal macrophages and dendritic cells (DCs) from control mice.
SD: standard diet group (control) (n = 6); SD+B: standard diet group receiving a daily dose of 5.0×108 CFU B. uniformis CECT 7771 by gavage for 7 weeks (n = 6); HFD: high fat diet group (n = 6); HFD+B: high fat diet group receiving a daily dose of 5.0×108 CFU B. uniformis CECT 7771 by gavage for 7 weeks (n = 6). In the cytokine production study, peritoneal macrophages (Figure 8A) and DCs (Figure 8B) were stimulated with stool stimuli. Non-stimulated peritoneal macrophages and DCs were evaluated as controls of basal cytokine levels. Figure 8A: TNF- α and IL-10 cytokines produced by stool-stimulated peritoneal macrophages; Figure 8B: TNF- α and IL-10 cytokines produced by stool-stimulated CDs. Data are expressed as means ± SD of duplicate measures determined in two independent experiments. Statistically significant differences of data are established at P<0.05.