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. 2012 Jul 26;7(7):e42048. doi: 10.1371/journal.pone.0042048

Table 1. Antibodies and fluoroprobes used in this study.

Target Antibody clone Manufacturer/Cat # Fluoroprobe Secondary antibody Fluoroprobe
Nucleus ---- DAPI ---- -----
8, 18, 19 cytokeratins CK3-6H5 Miltenyi Biotec 130-080-101 FITC ---- -----
8, 18, 19 cytokeratins CK3-6H5 Miltenyi Biotec 130-080-101 AF488
CD45 H130 BD Pharmingen 555480 AF594, custom conjugated
EpCAM Polyclonal, rabbit AB71916 ---- Anti-Rabbit AF647
Vimentin C-20, goat or polyclonal, goat Santa Cruz Biotechnology SC-7557 or Abcam ab11256 ---- Rabbit, anti-goat AF555
Rabbit, anti-goat AF647
EGFR polyclonal, rabbit Abcam ab2430 ---- Donkey, anti-rabbit AF633
CD44 G44-26, mouse BD Pharmingen 559942 APC ----- ----
N-cadherin polyclonal, rabbit Abcam ab12221 ---- Donkey, anti-rabbit AF633