Table 1. Antibodies and fluoroprobes used in this study.
Target | Antibody clone | Manufacturer/Cat # | Fluoroprobe | Secondary antibody | Fluoroprobe |
Nucleus | ---- | DAPI | ---- | ----- | |
8, 18, 19 cytokeratins | CK3-6H5 | Miltenyi Biotec 130-080-101 | FITC | ---- | ----- |
8, 18, 19 cytokeratins | CK3-6H5 | Miltenyi Biotec 130-080-101 | AF488 | ||
CD45 | H130 | BD Pharmingen 555480 | AF594, custom conjugated | ||
EpCAM | Polyclonal, rabbit | AB71916 | ---- | Anti-Rabbit | AF647 |
Vimentin | C-20, goat or polyclonal, goat | Santa Cruz Biotechnology SC-7557 or Abcam ab11256 | ---- | Rabbit, anti-goat | AF555 |
Rabbit, anti-goat | AF647 | ||||
EGFR | polyclonal, rabbit | Abcam ab2430 | ---- | Donkey, anti-rabbit | AF633 |
CD44 | G44-26, mouse | BD Pharmingen 559942 | APC | ----- | ---- |
N-cadherin | polyclonal, rabbit | Abcam ab12221 | ---- | Donkey, anti-rabbit | AF633 |