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. 2012 Jul 26;7(7):e41790. doi: 10.1371/journal.pone.0041790

Figure 3. Verification of the microarray results by semi-quantitative RT-PCR.

Figure 3

(A) RT-PCR results of the genes. The cDNA of ‘Meiwa’ and ‘Newhall’ leaves sampled at 0 (designated as M0 and N0, respectively) and 5 (designated as M5 and N5, respectively) days post inoculation (DPI) was amplified with specific primers of the selected genes, using Actin as a control. (B) Comparison of the expression ratios (M5/M0 and N5/N0) between RT-PCR analysis and the microarray data. The expression ratios were calculated by quantifying the band density using the Quantity One software.