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. 2012 Jul 26;7(7):e41776. doi: 10.1371/journal.pone.0041776

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© 2012 Pirooznia et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Histogram showing relative fold change in expression level of apoptosis related target genes in flies expressing dTip60^E431Q A. Staged second instar larvae were used for cDNA preparation. RT-PCR reactions were carried out in triplicate and the fold change was calculated using the 2−ΔΔCT method using RP49 as control. (B) List of selected apoptosis related target genes identified by microarray analysis and validated in the dTip60^E431Q A line using quantitative RT-PCR.