. 2012 Jul 26;7(7):e41776. doi: 10.1371/journal.pone.0041776

Table 2. Developmental stage at which expression of the different transgenes induces lethality.

Transgenic fly lines^a	Developmental Stage of Lethality^b
	Ubiquitous expression^c	Pan-neuronal expression^d
Wild type (w¹¹¹⁸)	Not lethal	Not lethal
APP	Pupae/Adult	Pupae/Adult
APP dCT	Not lethal	Not lethal
dTip60 ^E431Q A	Late 3^rd instar	Late 3^rd instar*
APP; dTip60 ^E431Q A	Early 2^nd instar (hatching delayed by 24–48 hrs)	Early 2^nd instar*
APP dCT; dTip60 ^E431Q A	Late 3^rd instar	Late 3^rd instar*
dTip60 ^E431Q B	Late 3^rd instar	Late 3^rd instar
APP; dTip60 ^E431Q B	Early 2^nd instar (hatching delayed by 24–48 hrs)	Early 2^nd instar
APP dCT; dTip60 ^E431Q B	Late 3^rd instar	Late 3^rd instar
dTip60^WT lines A, B, C	Partially lethal	Partially lethal
APP; dTip60^WT lines A, B, C	Partially lethal	Partially lethal
APP dCT; dTip60^WT lines A, B, C	Partially lethal	Partially lethal

Ten female virgin flies homozygous for the indicated transgene or control w¹¹¹⁸ were crossed to seven males homozygous for the Gal4 driver. All crosses were carried out in triplicate at 25°C.

The crosses were monitored daily and the developmental stage at which lethality occurred was scored.

The 337-Gal4 was used to drive ubiquitous expression of transgenes.

The 179-Gal4 driver located on the X-chromosome was used to drive pan-neuronal expression of transgenes. * Neuronal expression of low expressing independent fly line dTip60 HAT mutant (dTip60^E431Q A) alone or in conjunction with APP/APP dCT induced lethality in a fraction of the respective F1 progeny at the indicated developmental stage while the remainder of F1 progeny did not exhibit any lethal effect.