Figure 1. Pipeline of screen for PA14 virulence-attenuated mutants in C. elegans.

The three screening steps for identification of P. aeruginosa PA14 virulence-attenuated mutants are outlined; details of the screens are presented in the Materials and Methods and the text. The number of mutants obtained after each round of screening, as well as those removed from the pool for various reasons, is shown. Note that the 313 mutants identified in the primary screen and the 180 from the secondary screen represent 294 and 170 unique genes respectively because some genes were represented by multiple mutants, and a small fraction of mutants were in intergenic regions (see text). In the tertiary screen a single mutant defined each gene.