Fig 2.

Growth of recombinant cells of R. jostii RHA1 in W medium at 30°C and 200 rpm. (A) The initial A₆₀₀ of the culture was adjusted to about 0.1, and the cells were grown in medium containing 30 g/liter d-xylose. Samples were taken every 12 h, and the A₆₀₀ was determined. ■, R. jostii XYLAB; •, R. jostii XYLA; ▲, R. jostii/pNV18. (B) Cells were grown in medium containing different contents from 20 g/liter to 80 g/liter of d-xylose. The cell biomass yield, indicated as the A₆₀₀, was detected at 120 h. xylA, R. jostii XYLA; xylAB, R. jostii XYLAB. (C) Recombinant R. jostii XYLAB bearing xylA and xylB from S. lividans TK23 was incubated on 4 g/liter d-xylose (■), and R. jostii/pNV18 harboring the empty plasmid pNV18 was grown in W medium containing 4 g/liter glucose (•).