Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| E. coli TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| S. lividans TK23 | Wild type, source of xylA and xylB | 20 |
| R. jostii RHA1 | Wild type | 37 |
| R. opacus PD630 | Wild type | 24 |
| R. jostii XYLA | R. jostii RHA1 bearing plasmid pXYLA | This study |
| R. jostii XYLAB | R. jostii RHA1 bearing plasmid pXYLAB | This study |
| R. jostii pNV18 | R. jostii RHA1 bearing plasmid pNV18 | This study |
| R. opacus XYLA | R. opacus PD630 bearing plasmid pXYLA | This study |
| R. opacus XYLAB | R. opacus PD630 bearing plasmid pXYLAB | This study |
| R. opacus pNV18 | R. opacus PD630 bearing plasmid pNV18 | This study |
| R. jostii ΔxylB1 | R. jostii RHA1 xylB1 knockout | This study |
| R. jostii ΔxylB2 | R. jostii RHA1 xylB2 knockout | This study |
| R. jostii ΔxylB1B2 | R. jostii RHA1 double knockout of xylB1 and xylB2 | This study |
| R. jostii ΔxylB1_xylA | R. jostii RHA1 xylB1 knockout bearing plasmid pXYLA | This study |
| R. jostii ΔxylB2_xylA | R. jostii RHA1 xylB2 knockout bearing plasmid pXYLA | This study |
| R. jostii ΔxylB1B2_xylA | R. jostii RHA1 double knockout of xylB1 and xylB2 bearing plasmid pXYLA | This study |
| Plasmids | ||
| pMAT-TAG | Ampr; E. coli expression vector, source of tac promoter | Sigma |
| pNV18 | Kmr; E. coli-Rhodococcus shuttle vector | 10 |
| pTACHis18 | Kmr; expression vector containing the tac promoter, derived from pNV18 | This study |
| pXYLA | Kmr; pTACHis18 containing xylA from S. lividans TK23 | This study |
| pXYLB | Kmr; pTACHis18 containing xylB from S. lividans TK23 | This study |
| pXYLAB | Kmr; pTACHis18 containing xylA and xylB genes | This study |
| pK18mobsacB | Kmr; sacB; cloning vector for mobilization into bacteria | 45 |
| pKXYLB1 | Kmr; pK18mobsacB containing the xylB1 fragment | This study |
| pKXYLB2 | Kmr; pK18mobsacB containing the xylB2 fragment | This study |
| pKB1-SalI | Kmr; pKXYLB1 containing disrupted xylB1 | This study |
| pKB2-PstI | Kmr; pKXYLB2 containing disrupted xylB2 | This study |
a
Ampr, ampicillin resistance; Kmr, kanamycin resistance; sacB, gene encoding levansucrase.