Table 3.
Lipid content of R. jostii RHA1, R. opacus PD630, and their recombinants grown in W medium containing glucose or xylose under nitrogen-limited conditions
| Fatty acid or lipid | % lipid contenta |
|||
|---|---|---|---|---|
| PD630 (glucose) | PD630 XYLAB (xylose) | RHA1 (glucose) | RHA1 XYLAB (xylose) | |
| Fatty acid (total) | 61.78 ± 1.2 | 68.30 ± 2.9 | 42.63 ± 1.5 | 52.5 ± 3.0 |
| Myristic acid (C14:0) | 1.68 ± 0.2 | 0.92 ± 0.1 | 1.66 ± 0.1 | 1.38 ± 0 |
| Pentadecanoic acid (C15:0) | 6.63 ± 0.4 | 11.61 ± 1.2 | 6.89 ± 0.1 | 9.79 ± 0.2 |
| Palmitic acid (C16:0) | 25.92 ± 1.0 | 21.54 ± 1.7 | 25.33 ± 2.3 | 21.2 ± 1.7 |
| Palmitoleic acid (C16:1n7) | 5.50 ± 0.9 | 4.37 ± 1.3 | 4.91 ± 1.6 | 4.71 ± 1.1 |
| Heptadecanoic acid (C17:0) | 14.82 ± 2.3 | 19.62 ± 1.8 | 19.38 ± 0.7 | 22.39 ± 0.8 |
| Heptadecenoic acid (C17:1n7) | 14.32 ± 0.3 | 18.31 ± 0.6 | 14.37 ± 1.9 | 19.37 ± 0.9 |
| Stearic acid (C18:0) | 5.24 ± 0.1 | 3.38 ± 0.1 | 6.05 ± 0.8 | 3.33 ± 0.2 |
| Oleic acid (C18:1n9) | 17.85 ± 1.5 | 10.60 ± 0.9 | 13.8 ± 1.0 | 7.89 ± 0.7 |
| Linoleic acid (C18:2n6) | 1.78 ± 0.1 | 1.65 ± 0 | 1.47 ± 0.3 | 1.17 ± 0.1 |
a
Total fatty acids are expressed as a percentage of cell dry weight (CDW), and the fatty acid composition profile is shown as percent (wt/wt). The data were calculated from triplicate measurements, and data are represented as the average ± standard deviation. The carbon source is indicated in parentheses after the strain name.