Rank analysis for physical dissociation method (PDM) (A) and extraction solution (B). Block plots show mean percentage of recovery averaged across all three organisms for each extraction method. TV, touch vortexing; S1, sonication for 1 min; S2, sonication for 2 min; S5, sonication for 5 min; V2, vortexing for 2 min; V10, vortexing for 10 min; S1V1, sonication and then vortexing for 1 min each; S2V2, sonication and then vortexing for 2 min each; BB, Butterfield's buffer; BBT, Butterfield's buffer with 0.04% Tween 80; PBS, phosphate-buffered saline; PBST, phosphate-buffered saline with 0.04% Tween 80; MRD, maximum recovery diluent.