Fig 5.
KgtP is required for wild-type levels of OsIDH expression in rice. RNAs were isolated from rice leaves that were infiltrated with cultures of X. oryzae pv. oryzae PXO99A, PΔkgtP, and PΔhrcU strains for 8 h. (A) Semiquantitative RT-PCR analysis. The OsIDH mRNA level of tested strains was determined with the primer OsIDH-F/R (see Table S1 in the supplemental material). PCR products were then electrophoretically separated on a 1% agarose gel. The EF1-a gene of rice was used as an internal standard control. (B) Real-time quantitative RT-PCR analysis. The relative mRNA level of the OsIDH genes in rice inoculated with PΔkgtP was calculated with respect to the level of the corresponding transcripts in rice inoculated with wild-type PXO99A. Values given are the means ± standard deviations of triplicate measurements from a representative experiment. The experiment was repeated at least three times, and similar results were obtained.