Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Escherichia coli | ||
| DH5α | F− ϕ80dlacZ ΔM15 Δ(lacZYA-argF)U169 endA1 deoR recA1 hsdR17(rK− mK+) phoA supE44 λ− thi-l gyrA96 relA1 | Clontech |
| Agrobacterium tumefaciens | ||
| EHA105 | Rifr | This lab |
| X. oryzae pv. oryzae | ||
| PXO99A | Wild type, Philippine race 6 | This lab |
| PΔhrcU | hrcU deletion mutant of PXO99A | This lab |
| PΔhrpX | hrpX deletion mutant of PXO99A | This lab |
| PΔhrpG | hrpG deletion mutant of PXO99A | This lab |
| PΔhrpF | hrpF deletion mutant of PXO99A | This lab |
| PΔhpaB | hpaB deletion mutant of PXO99A | This lab |
| PΔhpaP | hpaP deletion mutant of PXO99A | This lab |
| PΔhrpE | hrpE deletion mutant of PXO99A | This lab |
| PΔ kgtP | kgtP deletion mutant of PXO99A | This study |
| CPΔkgtP | PΔkgtP containing pCkgtP; Kmr | This study |
| Plasmids | ||
| pMD18-T | pUC ori, cloning vector; Apr | TaKaRa |
| pUFR034 | incW mob(p) mob+ lacZa+; PK2 replicon, cosmid; Kmr | This lab |
| pHM1 | Broad-host-range vector, cos parA incW derivative of pRI40; Spr Smr | This lab |
| pKMS1 | Suicide vector derived from pK18mobGII, sacB+; Kmr | 71 |
| pEGAD | lacZa+, contains GFP; Kmr | This lab |
| pKΔkgtP | 1,052-bp fusion of the left and right regions flanking kgtP locus in pKMS1; Kmr | This study |
| pCkgtP | 1,630-bp fusion of the kgtP promoter and kgtP gene in pUFR034; Kmr | This study |
| pKgtP-c-Myc | pHM1 expressing KgtP with c-Myc tag under the control of the kgtP promoter; Spr Smr | This study |
| pkgtPPGFP | 1,017-bp fusion of the kgtP promoter and the coding region for gfp in pUFR034; Kmr | This study |
| pZWavrXa10 | Coding region for avrXa10 fused to the lacZ promoter of pBluescript II KS(+); Apr | This lab |
| pAvrXa10 | Coding region for avrXa10 with native promoter in pUFR034; Kmr | This lab |
| pΔ28AvrXa10 | Truncated avrXa10 missing the region corresponding to the N-terminal 28 amino acids; under the control of avrXa10 native promoter in pUFR034; Kmr | 27 |
| pNKgtPΔ28AvrXa10 | pUFR034 expressing the N-terminal 50 amino acids of KgtP and Δ28AvrXa10 under the control of the kgtp promoter; Kmr | This study |
| pKgtP-GFP | pEGAD expressing KgtP; Kmr | This study |
| pKgtPΔ6-GFP | pEGAD expressing 780 bp corresponding to the N terminus of KgtP; Kmr | This study |
| pKgtPΔ12-GFP | pEGAD expressing 66 and 72 bp corresponding to regions from the N and C termini of KgtP; Kmr | This study |
| pXopR-GFP | pEGAD expressing XopR; Kmr | This study |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance; Rifr, rifampin resistance; Spr, spectinomycin resistance.