Table 1.
Doubling times and final optical densities of wild-type strain DL1 and various mutantsa
| Strain | Glucose |
Galactose |
Lactose |
|||
|---|---|---|---|---|---|---|
| Avg Td (min) ± SD | OD600 | Avg Td (min) ± SD | OD600 | Avg Td (min) ± SD | OD600 | |
| DL1 (wild type) | 57.4 ± 6.7 | 1.08 | 67.5 ± 2.1 | 1.17 | 67.1 ± 4.3 | 0.97 |
| ΔEIILac | 57.5 ± 4.4 | 1.07 | 70.0 ± 4.2 | 1.13 | ND | 0.15 |
| ΔlacG | 72.4 ± 2.6 | 1.07 | 113.2 ± 7.9 | 1.16 | ND | 0.09 |
| ΔlacA1B1 | 61.9 ± 1.2 | 1.03 | 201.0 ± 18.6 | 0.23 | ND | 0.20 |
| lacT(M8stop) | 64.8 ± 1.9 | 1.12 | 74.9 ± 0.4 | 1.16 | ND | 0.19 |
| ΔEIIGal | 68.5 ± 8.4 | 0.86 | 107.6 ± 26.2 | 1.15 | 94.2 ± 6.5 | 0.88 |
| ΔlacR | 57.8 ± 1.5 | 1.16 | 64.1 ± 0.9 | 1.23 | 60.9 ± 2.3 | 0.93 |
| ΔlacA2B2 | 57.0 ± 2.3 | 1.05 | 66.9 ± 2.5 | 1.27 | 69.4 ± 1.2 | 0.95 |
| ΔlacA1B1 ΔlacA2B2 | 55.7 ± 0.7 | 1.01 | ND | 0.10 | ND | 0.13 |
| ΔEIIABMan | 67.9 ± 1.7 | 0.89 | 109.7 ± 5.2 | 1.17 | 67.2 ± 3.0 | 0.77 |
| ΔEIIGal/ΔEIIABMan | 61.4 ± 1.2 | 0.90 | 254 ± 47 | 0.59 | 61.2 ± 5.1 | 0.76 |
a
Results (averages ± standard deviations) are each based on three manual growth curve analyses using TV medium containing the specified carbohydrate (0.5%) in the presence of 5% of CO2. ND (not determined) is denoted when a strain displayed minimal growth and when the doubling time (Td) was too large for accurate calculations.