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. 2012 Aug;78(16):5724–5733. doi: 10.1128/AEM.00949-12

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Fig 4 — SDS-PAGE (12%) of membrane fractions of E. coli W3110 carrying different plasmids. pCom10 (lane 1) and pCom10_alkL (lanes 2) served as negative and positive controls, respectively, whereas strains containing pGEc47 (lane 3), pGEc47ΔB/pBT10 (lane 4), and pBTL10 (lane 5) showed low but significant alkL expression, which was absent with pBT10 (lane 6). Lanes M, PageRuler SM26614 (Fermentas GmbH, St. Leon-Rot, Germany). The arrow indicates AlkL (23 kDa), whereas AlkB prominently runs at ∼40 kDa.