Table 3.
Maximal DAME oxygenation activities of resting E. coli W3110 (pBT10) cells in the presence of different surfactants and solvents in a two-liquid-phase reactor setup
| Parameter or additive | Setting or concn | Relative maximal hydroxylation activitiesa |
|---|---|---|
| Parameters | ||
| Cell density | 20 gcdw liter−1 | 0.9 |
| Stirring speed | 3,000 rpm | 1.0 |
| Phase ratio | 1:2 (organic/total vol) | 1.0 |
| Additives | ||
| Rhamnolipids | 0.0001% (wt/vol) | 0.8 |
| 0.001% (wt/vol) | 1.0 | |
| 0.01% (wt/vol) | 1.0 | |
| DMSO | 1% (vol/vol) | 1.0 |
| 2% (vol/vol) | 0.3 | |
| Triton X-100 | 0.05 (vol/vol) | 1.0 |
| EDTA | 1 mM | 1.5 |
| 2.5 mM | 2.8 |
a
For each biotransformation, maximal activities are normalized to the maximal activity achieved with untreated cells in a parallel experiment (see the text for details).