Table 4.
NAME and DAME oxygenation activities of different alkBGT-expressing Pseudomonas strains
| Strain | Oxygenation activity (U gcdw−1)a |
|
|---|---|---|
| NAME | DAME | |
| P. putida KT2440 (pBT10) | 2.2 | 0 |
| P. putida GPo1 | 18 | 1.9b |
| P. putida GPo12 (pBT10) | 0 | 0 |
| P. fluorescens ATCC15453 (pBT10) | 3.3 | 1.8b |
| P. fluorescens CHA0 (pBT10) | 21 | 5.2 |
| Pseudomonas sp. strain VLB120 (pBT10) | 57 | 0.9 |
a
Calculated for the first oxygenation step based on product formation by resting cells: cell concentration, 1.0 gcdw liter−1; initial substrate concentration, 2.5 mM; reaction time, 5 min.
b
Reaction time, 15 min.