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. 2012 Aug;78(16):5724–5733. doi: 10.1128/AEM.00949-12

Table 5.

Activities of different recombinant E. coli W3110 strains expressing alkBGT and optionally alkL for NAME, DAME, octane, and nonane oxygenation

Plasmid Expression level of:
 Maximal sp acta (U gcdw−1)
AlkBGT AlkL NAMEb DAMEb Octanec Nonane
pBT10 High 104 1.9 24 2.4d
pGEc47 Low Low 71 31 54 72e (37)
pBT10;GEc47ΔB High Low 125 23 77 88e (46)
pBTL10 High High 128 53 100 97e (40)
a

Calculated for the first oxygenation step based on product formation by resting cells; for nonane, substrate consumption also was considered (see Materials and Methods).

b

Cell concentration, 1.0 gcdw liter−1; initial substrate concentration, 2.5 mM; reaction time, 5 min.

c

Cell concentration, 0.5 gcdw liter−1; initial substrate concentration, 1 mM; reaction time, 6 min.

d

Cell concentration, 1.0 gcdw liter−1; initial substrate concentration, 1 mM; reaction time, 6 min.

e

Cell concentration, 0.5 gcdw liter−1; initial substrate concentration, 1 mM; reaction time, 6 min; activities based on substrate depletion as well as product formation (in parentheses) are given.