Table 2.

Analysis of narG, nirK, nirS, and nosZ derived from palsa peat soil based on two distinct threshold similarities utilized for calling OTUs

Gene marker (read)	Threshold similarity (%)	Soil layer (cm)	No. of sequences	Good's coverage (%)a	No. of OTUs observed	No. of OTUs estimatedb	Hc	Ed
narG (forward)	67	0 to 20	2,956	100.0	4	4 (4–4)	0.56	0.28
		>20	1,925	100.0	3	3 (3–3)	0.81	0.51
	97	0 to 20	2,956	92.1	165	284 (232–375)	3.39	0.46
		>20	1,925	94.7	67	157 (103–293)	2.21	0.36
narG (reverse)	67	0 to 20	3,541	100.0	2	2 (2–2)	0.01	0.01
		>20	1,774	100.0	1	1 (1–1)	0.00	n.a.
	97	0 to 20	3,541	97.0	195	417 (325–576)	3.22	0.42
		>20	1,774	97.9	73	117 (92–172)	2.47	0.40
nirK	83	0 to 20	8,313	100.0	4	4 (4–4)	1.49	0.75
		>20	3,299	99.9	8	9 (8–16)	0.71	0.24
	97	0 to 20	8,313	99.9	34	40 (35–61)	2.33	0.46
		>20	3,299	99.4	48	67(54–105)	1.72	0.31
nirS	82	0 to 20	85	100.0	4	4 (4–4)	0.78	0.25
		>20	833	99.6	12	15 (12–37)	2.39	0.75
	97	0 to 20	85	97.5	7	8 (7–15)	1.09	0.39
		>20	833	97.4	46	85 (59–159)	3.36	0.61
nosZ (forward)	80	0 to 20	1,563	99.9	5	5 (5–5)	1.30	0.56
		>20	1,044	99.9	6	6 (6–6)	1.47	0.58
	97	0 to 20	1,563	99.5	32	38 (33–59)	2.24	0.45
		>20	1,044	99.3	25	29 (26–44)	2.37	0.51
nosZ (reverse)	80	0 to 20	1,856	100.0	4	4 (4–4)	1.27	0.63
		>20	1,381	99.9	6	6 (6–6)	1.45	0.56
	97	0 to 20	1,856	99.6	29	33 (30–51)	2.03	0.42
		>20	1,381	99.5	27	32 (28–55)	2.32	0.49

^aPercent library coverage (Good's coverage): C = (1 − n_s/n_t) × 100, where n_s is the number of OTUs that occur only once and n_t is the total number of sequences.

^bChao1 richness estimate with upper and lower 95% confidence intervals given in parentheses.

^cShannon diversity index.

^dSpecies evenness.