Table 4.

Copy numbers of denitrification-associated genes in palsa peat soil

Gene	Copy no. by soil layer
	From 0–20 cm		Below 20 cm
	Per 16S rRNA gene (%)a,g	Per ng of DNA	Per 16S rRNA gene (%)b,g	Per ng of DNA
narGc	(8.9 ± 0.5) × 10−1	(1.5 ± 0.1) × 104	(4.9 ± 0.4) × 100	(5.1 ± 0.2) × 104
nirKd	(1.2 ± 0.3) × 10−3	(1.4 ± 0.3) × 100	(3.3 ± 0.9) × 10−4	(8.6 ± 2.3) × 10−1
nirSe	(1.6 ± 0.2) × 10−1	(2.5 ± 0.3) × 102	(1.4 ± 0.3) × 10−2	(3.7 ± 0.7) × 101
nosZf	(2.7 ± 0.5) × 10−3	(4.3 ± 0.8) × 101	(8.4 ± 1.0) × 10−3	(8.8 ± 0.8) × 101

^a16S rRNA gene copy numbers were (1.6 ± 0.1) × 10⁶ per ng DNA.

^b16S rRNA gene copy numbers were (1.0 ± 0.1) × 10⁶ per ng DNA.

^cAssay validation for narG: detection limit, 10¹ copies; PCR efficiency, 108.2%; standard curve r², 0.993.

^dAssay validation for nirK: detection limit, 10⁰ copies; PCR efficiency, 106.0%; standard curve r², 0.993.

^eAssay validation for nirS: detection limit, 10¹ copies; PCR efficiency, 97.5%; standard curve r², 0.993.

^fAssay validation for nosZ: detection limit, 10¹ copies; PCR efficiency, 101.5%; standard curve r², 0.989.

^gAssay validation for 16S rRNA: detection limit, 10¹ copies; PCR efficiency, 95.9%; standard curve r², 0.994.