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. 2012 Aug;78(16):5690–5698. doi: 10.1128/AEM.00523-12

Fig 5.

Fig 5

Characterization of partially purified H. zea ALP. Partially purified HzALP samples (2 μg to 5 μg) were silver stained for total protein (A) or transferred to PVDF filters and probed with antisera against ALP of A. gambiae (B), antisera against 130-kDa APN from H. virescens (C), biotinylated Cry1Ac alone (D), or biotinylated Cry1Ac and a 500-fold excess of unlabeled Cry1Ac (E). Arrow indicates the position of the HzALP protein band for reference.