Fig 4.

SDS-PAGE gel of various MBP–WS/DGAT–His-tagged fusion proteins isolated using the rapid two-step purification procedure described in Materials and Methods. Samples are flanked by a protein standard (New England Biolabs) on either side. A prominent band is seen for each sample corresponding to the complete fusion protein, and various degrees of degradation or premature translational termination are found for several of the proteins shown. Acronyms for the enzymes reported are defined in Materials and Methods.