Fig 6.

Rates of specific activity plotted as a percentage of the specific activity found using palmitoyl-CoA (C₁₆-CoA). Assays were run with 20 nmol of dodecanol and 15 nmol of various fatty acyl-CoA substrates in a final volume of ∼1.2 ml of buffer, and the specific activity was monitored spectrophotometrically at 412 nm. Quantities of protein added ranged from 0.3 to 4.5 μg based on the specific activity results obtained with dodecanol and palmitoyl-CoA for each protein, as listed in Table 1.