Table 2.
Inoculation | Population in the phyllosphere, log(CFU/g)a |
Disease indexb | Gene annotation | Identifierc | Corresponding gene in Sphingomonas sp. S17d | ||
---|---|---|---|---|---|---|---|
Sphingomonas sp. Fr1 |
P. syringae DC3000, 21 dpi | ||||||
0 dpi | 21 dpi | ||||||
Fr1 (wild type) | 8.0 ± 0.2 | 8.3 ± 0.4 | 8.1 ± 1.1 | 0.0 | |||
17DF5 | 7.6 ± 0.6 | 7.0 ± 0.8 | 8.6 ± 0.7 | 0.3 | Cytochrome o ubiquinol oxidase subunit II | 2,537 | SUS17_373 |
17CC8 | 7.0 ± 0.3 | 6.4 ± 0.7 | 8.6 ± 0.9 | 0.4 | Intergenic | 1,536/1,537 | —e |
16BG1 | 7.3 ± 0.5 | 8.1 ± 0.9 | 8.3 ± 1.4 | 0.4 | Mo cofactor biosynthesis protein C | 990 | SUS17_830 |
4JA3 | 5.7 ± 0.7 | 6.3 ± 1.0 | 9.1 ± 0.6 | 0.5 | Sensor histidine kinase | 1,140 | SUS17_272 |
4CA8 | 7.4 ± 0.3 | 6.9 ± 0.6 | 9.5 ± 0.4 | 0.6 | Putative membrane protein | 1,490 | SUS17_2491 |
17EH7 | 7.5 ± 1.0 | 7.8 ± 0.7 | 8.9 ± 0.8 | 0.7 | Cytochrome o ubiquinol oxidase subunit IV | 2,534 | SUS17_370 |
2BB5 | 7.0 ± 1.2 | 7.5 ± 0.7 | 9.5 ± 0.7 | 0.7 | GTP pyrophosphokinase (SpoT) | 155 | SUS17_3068 |
16EC12 | 7.7 ± 0.3 | 6.7 ± 0.6 | 9.4 ± 0.7 | 0.7 | Putative membrane protein | 1,490 | SUS17_2491 |
5BC11 | 8.4 ± 0.4 | 8.2 ± 0.5 | 9.3 ± 0.5 | 0.7 | Cytochrome o ubiquinol oxidase subunit II | 2,537 | SUS17_373 |
16AB2 | 6.5 ± 0.7 | 7.4 ± 0.7 | 9.1 ± 0.6 | 0.8 | Multimodular transpeptidase-transglycosylase | 869 | SUS17_3528 |
Axenicf | 9.4 ± 0.4 | 1.0 |
Sphingomonas sp. Fr1 and P. syringae DC3000 populations in the phyllosphere at 0 and 21 days postinfection. Shown are means ± standard deviations of log-transformed CFU per gram of leaf fresh weight (n = 12).
Disease severity at 20 dpi. Each plant was given a rank of 1 to 5 (healthy to diseased). Shown are normalized data for the weighted arithmetic means (n = 16 to 24).
Gene identifier according to a RAST annotation of a Sphingomonas sp. Fr1 draft genome (unpublished).
From reference 19.
—, no microsynteny in Sphingomonas sp. S17.
Control plants that were grown axenically prior to pathogen application.