Fig 5.

Influence of the rpoN::aacC3 mutation on expressions of genes associated with an RpoN binding site. (A) Qualitative standardized RT-PCR expression analysis of genes (in the wild-type strain or the rpoN::aacC3 mutant) associated with an RpoN binding site. Cells were grown in the absence (−) or in the presence (+) of As^III or under conditions with or without N. For each gene, RT-PCR amplicon volumes loaded into the gel were standardized. (B) β-Galactosidase activity (in Miller units) derived from the transcription of the phoB1::lacZ and pstS1::lacZ reporters in the wild type and the rpoN::aacC3 mutant and as a function of cells grown in the presence or absence of 100 μM As^III.