Fig 5.

Circularization of the aac(2′)-IIa and hypothetical genes (bglu_5091g13). (A) Generation of the closed circular form of the aac(2′)-IIa and bglu_5091g13 genes at 36- to 37-bp direct repeats (att). PCR primer positions are shown under the gene map. (B) Detection of the circularization by PCR. (C) Comparison of the 36- to 37-bp direct repeats in B. glumae 5091 with other similar sequences in the IncP islands from K. pneumoniae 342 and Brucella suis 1330. The open boxes indicate the translational initiation codon of the aac(2′)-IIa gene. The gray boxes indicate the putative Shine-Dalgarno (SD) sequence of the aac(2′)-IIa gene. Underlined letters show differences in nucleotides in the direct repeats.