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. 2012 Jul 26;8(7):e1002840. doi: 10.1371/journal.pgen.1002840

Figure 7. mPINC interacts with PRC2 in HC11 cells.

Figure 7

(A–D) RIP assays were performed with HC11 cells using antibodies to PRC2 members including, EZH2, SUZ12 and RbAp46. An MLL1 antibody was used as a negative control and an lncRNA known to interact with PRC2, Tug1, was used as a positive control. RNA was isolated from pull downs to detect associated RNAs. (A) RT-PCR shows mPINC is associated with PRC2 members, but not MLL1. (B–D) qPCR shows the amount of Tug1 (B), mPINC1.0 (C) and mPINC1.6 (D) transcript associated with each protein as a percentage of input RNA levels. Data represent mean ± SD (n = 3).