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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Anesthesiology. 2012 Aug;117(2):381–388. doi: 10.1097/ALN.0b013e3182604b2b

Fig. 2. ErbB2 tyrosine kinase inhibitor reduces mechanical allodynia.

Fig. 2

Whisker pad mechanical allodynia is alleviated by an active ErbB2 tyrosine kinase inhibitor, Lapatinib, in wild type (WT) rats with chronic constriction injury of infraorbital nerve (CCI-ION). In week 4 post CCI-ION, mechanical threshold in wild type rats was tested to confirm mechanical allodynia to von Frey filaments. Lapatinib was administered by intraperitoneal injection (vehicle, 0.2, 1 and 5mg/kg) after the pre-drug threshold was determined on both whisker pads. Bilateral behavioral responses to mechanical stimuli were monitored 30min, 1h, 3h and 6h post Lapatinib administration. (A) Lapatinib dose-dependently (Dimethyl sulfoxide (DMSO), 0.2, 1 and 5mg/kg) decreased mechanical allodynia in wild type rats with CCI-ION at 3h after drug administration. **p<0.01 at 1mg/kg, ***p<0.001 at 5mg/kg vs. DMSO. (mean±S.D) (B) Mechanical thresholds were increased by Lapatinib administration on the nerve injured side at 30min, 1h and 3h, with a maximal effect found at 3h post. *p<0.05 at 1h, ***p<0.001 at 3h vs. pre-drug. There was no change in mechanical threshold on the contralateral side after intraperitoneal injection of Lapatinib. (mean±S.D)