Fig. 2.

DHHC3 palmitoylation specificity among integrin subunits. PC3 cells with vector (V) and DHHC3 (D3) stable knockdown were labeled with [³H]palmitate for 2 h. Then α3, β1, α6, β4 integrin and CD9 proteins were immunoprecipitated and visualized by [³H]palmitate labeling (lanes 1–8, 11,12). In the second panel (lanes 9, 10), β1 immunoprecipitation was done after depletion of α3 from the lysates, leaving α6β1. The far right panel shows unidentified [³H]palmitate labeled proteins in the whole-cell lysate