Fig. 3.

DHHC3 affects integrin-dependent cable formation and signaling in PC3 cells. a PC3 cells with vector (vector KD) and DHHC3 (DHHC3 KD) knockdown were plated on polymerized Matrigel in complete growth medium in 24-well plates. After 24 h, photographs were taken and mean % cable formation was quantitated (n = 3). b PC3 cells with vector knockdown (V) and DHHC3 knockdown (D3) were incubated with control mouse IgG, with rat anti-α6 mAb, or with mouse anti-β4 mAb for 1 h at 4°C. Cells coated with primary antibody were then plated in wells coated with goat-anti-mouse or goat-anti-rat IgG, and incubated for 30 min at 37°C, to trigger integrin signaling. After cell lysis, proteins were separated and blotted for the indicated signaling molecules. Density units in the bar graph represent p-Src(416)/Total c-Src (from top two panels). The bottom two panels show an actin loading control and confirm DHHC3 knockdown