Fig. 5.

Pepstatin A restores β4 integrin expression after DHHC3 ablation. a PC3 cells with vector control (V) or DHHC3 stable knockdown (D3) were treated with the indicated protease inhibitors or mock-treated (M) for 24 h. After cell lysis, β4 integrin expression was detected by blotting. Numbers represent % of β4 integrin expression relative to vector knockdown cells. b PC3 cells with vector and DHHC3 stable knockdown (2 independent samples) were treated with or without Pepstatin A for 24 h before labeling with [³H]palmitate for 2 h. β4 integrin was immunoprecipitated and then expression of β4 (top panel) was detected by blotting, and by [³H] labeling (second panel). Normalized β4 palmitoylation/protein was determined (bottom panel). c Shown are many unidentified [³H]palmitate-labeled proteins from PC3 whole cell lysate (top panel) and immunoblotting for DHHC3 protein (bottom panel)