Figure 4.

Obesity induces IL-17 in the CIA mice. (A) The expression of IL-17 in the splenocytes obtained from each mouse was determined by real time PCR. The data is expressed as the mean ± SD. ^*P < 0.05, ^**P < 0.01 compared with the CIA mice. (B) The splenocytes from the mice of each group were cultured with 25 ng/ml PMA and 250 ng/ml Ionomycin for 4 h. The expression of IL-17 was determined by intracellular flow cytometry analysis. The data is representative of three independent experiments. (C) Immunohistochemical assessment of IL-17 expression in mice joint with CIA and obese (original magnification, 400×).