Figure 1.
No synergistic effect of adiponectin plus IL-1β on the production of VEGF, MMP-1, or MMP-13 in RA FLSs. Synovial cells (2.5 × 105 cells/60 mm dish/2 ml serum-free media) were stimulated with IL-1β for 24 h at 10 or 100 pg/ml in the absence or presence of 10 µg/ml adiponectin, as described in the Materials and Methods section. The levels of VEGF, MMP-1, and MMP-13 were measured by ELISA from culture supernatants. Three independent experiments were performed in quadruplicate with FLSs from each patient. The data shown are representative of three independent experiments, and similar results were obtained from all three. Values are expressed as mean ± S.E.M.