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. 2012 Jul 27;6:47. doi: 10.3389/fncir.2012.00047

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Copyright © 2012 Saunders, Johnson and Sabatini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Co-infection with DIO or DO/FAS rAAVs drives co-expression without spatial interference. (A) Infection of multiple DIO Cre-On rAAVs results in co-expression of transgenes without interference. Left, a sagittal section of a D2-Cre mouse infected with DIO-GFP and DIO-mCherry in the striatum. Right, normalized mean fluorescent values for GFP and mCherry quantified from boxed inset in A. (B–D) Infection of multiple Cre-Off rAAVs results in co-expression of transgenes without spatial interference. Left, sagittal sections from wild type mice infected with B. DO-mCherry + DO-GFP, C. DO-mCherry + FAS-GFP, or D. FAS-TdTomato + FAS-GFP in the striatum. Right, normalized mean fluorescent values for GFP and mCherry/TdTomato quantified from boxed inset in to the left.