Fig. 5.

Heart-infiltrating A.SW CD4⁺ T cells produce more interleukin (IL)-17A and less interferon (IFN)-γ compared to B10.S CD4⁺ T cells. (a) Representative bivariate dot-plots of IL-17A and IFN-γ staining from A.SW and B10.S mice. (b) Relative frequencies of IL-17A⁺ T helper type 17 (Th17) cells and IFN-γ⁺ Th1 cells as a proportion of CD4⁺-gated cells were analysed by flow cytometry. (c). The absolute counts of CD4⁺IL-17A⁺ and CD4⁺IFN-γ⁺ cells were analysed by flow cytometry. (d) IL-23 was higher in the heart tissue of A.SW mice than B10.S by enzyme-linked immunosorbent assay (ELISA). Data are presented as the mean ± standard deviation. Statistics are by two-tailed Student's t-test. Data depict one representative experiment of three conducted with five mice per group.