Fig. 3.

Consistency of interferon (IFN)-γ responses in coeliac patients underwent two separate wheat challenges. Responsiveness to gliadin in 13 coeliac patients underwent a second short wheat consumption after 3–10 months of a strict gluten-free diet (wash-out). Peripheral blood mononuclear cells (PBMCs) were assayed by enzyme-linked immunospot (ELISPOT) for recognition of deamidated gliadin (a) or 33-mer (b) on day 0 and day 6 of oral challenge. Results are shown as in Fig. 1. Dashes indicate the median values of IFN-γ responses among patients (*P < 0·04).