Table 1.
Target sequence | Double strand DNA oligo sequence | |
IL-17RA shRNA 1 | CAAGTCCAAGACCATCTTA | 5'-ccggcaCAAGTCCAAGACCATCTTAttcaagagaTAAGATGGTCTTGGACTTGtgtttttg-3' |
5'-aattcaaaaacaCAAGTCCAAGACCATCTTAtctcttgaaTAAGATGGTCTTGGACTTGtg-3' | ||
IL-17RA shRNA 2 | CCAGCGATCCAATGTCACA | 5'-ccggcaCCAGCGATCCAATGTCACAttcaagagaTGTGACATTGGATCGCTGGtgtttttg-3' |
5'-aattcaaaaacaCCAGCGATCCAATGTCACAtctcttgaaTGTGACATTGGATCGCTGGtg-3' | ||
IL-17RA shRNA 3 | CAGCAGCCATGAACATGAT | 5'-ccggcaCAGCAGCCATGAACATGATttcaagagaATCATGTTCATGGCTGCTGtgtttttg-3' |
5'-aattcaaaaacaCAGCAGCCATGAACATGATtctcttgaaATCATGTTCATGGCTGCTGtg-3' | ||
IL-17RA shRNA 4 | GGAAGAAAGTGGAGTGGTA | 5'-ccgggaGGAAGAAAGTGGAGTGGTAttcaagagaTACCACTCCACTTTCTTCCtctttttg-3' |
5'-aattcaaaaagaGGAAGAAAGTGGAGTGGTAtctcttgaaTACCACTCCACTTTCTTCCtc-3' | ||
Random shRNA | TTCTCCGAACGTGTCACGT | 5'-ccggTTCTCCGAACGTGTCACGTttcaagagaACGTGACACGTTCGGAGAAtttttg-3' |
5'-aattcaaaaaTTCTCCGAACGTGTCACGTtctcttgaaACGTGACACGTTCGGAGAA-3' |
shRNA: Short hairpin RNA; IL-17RA: Interleukin-17A receptor.