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Primers and restriction enzymes used in PCR-RFLP assays for genotyping.
| Gene | Polymorphism | Primer sequence 5′→3′ | T (°C) | Size (bp) | Restriction enzyme |
|---|---|---|---|---|---|
| TGFB1 | C-509T | F: TTG AGT GAC AGG AGG CTG CTT A R: GCT GGG AAA CAA GGT AGG AGA A |
63 | 178 | Eco81I |
| T+29C | F: CCA CCA CAC CAG CCC TGT T R: TCC GCT TCA CCA GCT CCA T |
63 | 186 | MspA1I | |
| TGFBR2 | G-875A | F: GGA ATG TCT TGG GCA AAT CT R: ACC TGA ATG CTT GTG CTT TTA TT |
59 | 152 | TaaI |
F, forward primer sequence; R, reverse primer sequence.
