Table II.

The correlation between Pin1 and p53 protein expression and clinicopathological characteristics.

		Pin1 staining (IHC)			p53 staining (IHC)
Parameters	N	Positive	Negative	P-value	Positive	Negative	P-value
	110	74	36		63
Lymph node
Positive	65	54	11		40	25
Negative	45	20	25	0.000	23	22	0.277
Differentiation
Low	30	24	6		22	8
Middle	69	45	24		36	33
High	11	5	6	0.095	5	6	0.104
Stage
I	31	18	13		20	11
II	40	34	6		22	18
III + IV	39	29	10	0.038	21	18	0.626
TNM classification
T1	8	7	1		7	1
T2	67	55	12		35	32
T3	11	8	3		7	4
T4	24	19	5	0.847	14	10	0.275
T1+T2	75	62	13		42	33
T3+T4	35	27	8	0.492	21	14	0.693
Gender
Female	18	12	6		12	6
Male	92	70	22	0.484	50	42	0.260
Age
<49	17	12	5		11	6
50–59	18	12	6		11	7
60–69	46	28	18		26	20
>70	29	18	11	0.868	15	14	0.833

In each case, the intensity (weak, moderate or strong) and pattern (incomplete or complete) of nucleus and cytoplasm staining, and the percentage of neoplastic immunoreactive cells (cut-off of 10%) were evaluated. Tumors were scored as follows: score 0, no appreciable staining or staining in <5% neoplastic cells; score 1+, tumors with faint/barely appreciable incomplete nucleus and cytoplasm staining in 5–25% neoplastic cells; score 2+, tumors with weak to moderate complete nucleus and cytoplasm staining or containing 25–50% neoplastic cells with moderate incomplete nucleus and cytoplasm immunostaining; and score 3+, strong immunoreactivity of the entire nucleus and cytoplasm >50% neoplastic cells or containing >50% neoplastic cells with strong nucleus and cytoplasm immunoreactivity. Tumors classified as 0 or 1+ were considered ‘negative’ and those scored as 2+ or 3+ were classified as ‘positive’.