FIG. 5. .

MII eggs derived after TPEN exposure are developmentally competent. MII eggs obtained after transient TPEN exposure followed by a 14-h culture in IVM medium were parthenogenetically activated. A) Following parthenogenetic activation, the preimplantation developmental progression of eggs derived from TPEN-treatment (5h MT; gray bars), in vivo ovulation (black bars), and IVM (white bars) was scored. Specifically, the progression to pronuclear (PN), two-cell (2C), and blastocyst stage (B) were analyzed by light microscopy, and representative images are shown in B. The percentages of PN and 2C were calculated based on total number of MII eggs. The percentage of blastocysts was calculated based on number of 2C embryos. The experiment was performed three times and results represent the mean ± SEM. Between 20 and 40 eggs were used per group per experiment. For each embryo stage, groups with uncommon letters indicate statistical differences (P < 0.05). Bars = 40 μm.