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. 2012 Apr 25;87(1):23. doi: 10.1095/biolreprod.112.099747

FIG. 5. .

FIG. 5. 

DNA methylation at CpG (−955), CpG (−869), and CpG (−789) of the CYP19A1 promoter in Day 19 gonads of ZZ-male chicken embryos exposed to EE2 (20 μg/egg) from the indicated days of incubation. Orange curve and the right y-axis indicate degrees of morphological feminization shown in Figure 1B. Each datum point indicates DNA methylation at CpG sites 955, 869, or 789 nt upstream of the CYP19A1 TIS (mean ± SEM of five or more independent gonads). CpG methylation in vehicle-exposed ZZ-male testes and ZW-female ovaries are also shown. *P < 0.05 and **P < 0.01 compared to vehicle-exposed ZZ-male testes (t-test).